- Shanghai Rapeseed Database

Welcome to Shanghai RAPESEED Database!

MICROARRAY HYBRIDIZATION

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Addr:300 Fenglin Road Shanghai,200032 P.R China

rapeseed@sibs.ac.cn

When using the RAPESEED, please cite the following reference:
Wu, G.Z., Shi, Q.M., Niu, Y., Xing, M.Q., Xue, H.W. "Shanghai RAPESEED Database: a resource for functional genomics studies of seed development and fatty acid metabolism of Brassica. Nucleic Acids Research,2008,36: D1044-D1047; doi:10.1093/nar/gkm780

　　Click here to download the original microarray GPR files.
　　cDNA microarray (chip) is a powerful strategy for gene expression research. cDNA inserts of ESTs in our cDNA library were PCR amplified and used to generate the glass-based cDNA chip. The chip has 8192 spots, including 8095 Brassica Napus genes, 81 blanks and 16 negative controls. The chip is divided into 32 sub-matrix. There is 16*16 spots in every sub-matrix. The distance between two spots is 0.281 millimeter.
　　The spoting map is showed in the figure bellow:

　　Immature seeds at different developmental stages were used for hybridization. The gene expression profiles were attached to the search result of each gene.